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EST frequency :Number of supporting EST observations for the splice sites.

"Gene Name or Gene Alias": the gene symbol and gene name approved by NCBI(e.g. TNNT1, CD44).
"mRNA Accession & mRNA gi": mRNA Accession and mRNA gi approved by NCBI.
"Definition": the GenBank accession number. 
"Human and Mouse Cytoband": the cytogenetic location of a gene (e.g. 1p36, 1q20, etc).
"chromosome"
: Chromosome number (e.g. chromosome 22).
"
UniGene id": A unique UniGene identifier for mRNA/EST sequences. from GeneBank.

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All the search is case insensitive. 
Query against cytogenetic locations
Human: use the "n[p|q]n" format (e.g. 3p16, 22q12, etc)
Mouse: e.g. "9 20" means position 20 cM on chromosome #9

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